ALBERTO edgardo Omar
Solid-state bioprocessing of sugarcane bagasse with Auricularia fuscosuccinea for phenolic compounds extraction
CONIGLIO, ROMINA; DÍAZ, GABRIELA; LÓPEZ, CINTHYA; RESTELLI, MARÍA; GRASSI, EMANUEL; ALBERTÓ, EDGARDO; ZAPATA, PEDRO
PREPARATIVE BIOCHEMISTRY AND BIOTECHNOLOGY
TAYLOR & FRANCIS INC
Sugarcane bagasse is a natural source of phenolic compounds. However, these compounds are bound to lignocellulose components, reducing their ability to function as good antioxidants. These linkages are hydrolyzed by enzymes like β-glucosidases, increasing free phenolics. Auricularia is a food-grade genus capable of producing β-glucosidases. The aim of this work was (I) to determine naturally occurring species of Auricularia and (II) to obtain phenolic compounds through the solid-state bioprocessing of sugarcane bagasse. We have successfully isolated five strains that were assigned to the taxon A. fuscosuccinea. We determined β-glucosidase activity by fluorescence plate assay of the five isolated strains and adjusted an optimal temperature for mycelial growth at 30 °C. A. fuscosuccinea LBM 243 was chosen for solid-state bioprocessing of sugarcane bagasse. β-glucosidase activity (12.2 ± 0.62 U l−1) and protein content (51.58 ± 6.26 mg l−1) were highest on day 20 of culture. The maximum value of total phenolic content (507.5 ± 9.05 mg l−1) was obtained at day 20 and antioxidant capacity (34.44% ± 11.20) was highest at day 10, both in ethanolic extracts. The best performance of ethanol against methanol extraction in this work is highlighted considering ethanol to be a safe, efficient, and low-cost solvent.