Concentration of TBA. Reactive subtance in lung of castrated rats

CARGNELUTTI E.; OJEDA M.S.; GIMENEZ M.S.

Uspallata, Mendoza

Congreso; XXIII Reunión Científica Anual de la Sociedad de Biología de Cuyo-III Reunión Anual Sociedad Argentina de Microscopía; 2005

Sociedad de Biología de Cuyo

CONCENTRATION OF TBA-REACTIVE SUBTANCE IN LUNG OF CASTRATED MALE RAT Cargnelutti E, Ojeda MS, Gimemez MS. Bioquímica Molecular, Facultad de Química, Bioquímica y Farmacia, UNSL.5700 San Luis. E-mail: mgimenez@unsl.edu.ar Oxidative lung damage may be associated to destruction of alveolar cells. Thiobarbituric acid reactive substances (TBARS) were measured as marker of lipid peroxidation of alveolar ephitelial cells (AECs). The objective of our work was study if castration changes the concentration of lung TBARS of castrated male rat. Adult male rats weighed 200-250 g were separated in two groups: control (G1) and castrated (G2). 21 days after castration the rats were sacrificated under a light anesthesia with ether, the trachea was cannulated and the extracellular surfactant extracted with NaCl 0,5 M. After that, the lungs were extracted, washed and homogeneized in PBS solution pH 7,4. In one aliquot of lung homogenate the concentration of TBARS, through the synthesis of a red aduct between TBA (Thiobarbituric Acid) and MDA (Malonaldehyde) was measured. The results were expressed as nmol/g tissue; G1= 1,62 x 10 -4 ±0,03 and G2= 1,2x10 -4 ±0,01, p˂0,01, n=20. We think that castration increases the production of oxidative stress, what would cause alterations in lung morphology and lung metabolism previously found (Ojeda MS et al., 1998).