Cytokine production in mesenteric lymphs nodes induced by Yersinia enterocolitica infection: role of YopH

DÍAZ, MARÍA SOLEDAD; DABE, MABEL NOEMÍ; ELICABE, RICARDO JAVIER; CARGNELUTTI, ETHELINA; SILVA, JUAN EDUARDO; DI GENARO, MARÍA SILVIA

Potrero de los Funes

Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

Cytokine production in mesenteric lymph nodes INDUCED by Yersinia enterocolitica infection: role of YopH. Díaz MS, Dave MN, Eliçabe RJ, Cargnelutti E, Silva JE, Di Genaro MS. Laboratory of Immunopathology, National University of San Luis, IMIBIO-SL (CONICET-UNSL), sdigena@unsl.edu.ar.   Yersinia enterocolitica (Ye) is an enteropathogenic bacterium that invades Peyer patches (PP) and may disseminates to mesenteric lymph nodes (MLN), spleen (S), lung and liver. YopH  is a tyrosine phosphatase secreted by Ye that inhibits the phagocytosis and lymphocyte  activation.  The role of YopH during in vivo infection have yet to be completely elucidated. IFN-g plays a central role in the immune response against Ye. The aim was to compare IFN-g production in MLN after oral infection with Ye WA-314 (wild-type) or YeDyopH (YopH deficient). Moreover, we analized the production of IL-17, a cytokine associated with mucosal immune response. C57BL/6  mice were orally infected with 1?5 108 colony forming units (CFU) of Ye. The CFU in PP, MLN and S were determined 5 days after infection. Intracellular cytokine production was analysed in cells from MLN by flow cytometry. We detected lower CFU in PP and MLN after Ye DyopH infection (p<0.05). The median fluorescence intensity (MFI) of IFN-g+cells in MLN was higher after Ye WA-314 infection (p<0.05). No differences were detected in IL-17+cells. We conclude that YopH increases in vivo IFN-g production in MLN, but this cytokine may not compesate the YopH-mediated immune evasión.