Effect of TNFRPp55 deficiency on the inflammatory process exhibited at the end of pregnancy

ARIAS JL; RAGUSA JA; CARGNELUTTI E; CASAIS M; ANZULOVICH AC; DI GENARO MS

Potrero de los Funes, San Luis

Congreso; XLII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB-2011).; 2011

Sociedad Argentina de Investigación Bioquímica

CB-P05.EFFECT OF TNFRp55 DEFICIENCY ON THEINFLAMMATORY PROCESS EXHIBITED AT THE ENDOF PREGNANCYArias JL1, Ragusa JA2, Cargnelutti E1, Casais M3, Anzulovich AC2,Di Genaro MS1.1Lab of Immunopatholoy and Flow Cytometry, 2LABCRON,IMIBIO-SL,CCT CONICET San Luis, 3LABIR, UNSL. E-mail:jlarias1980@gmail.comTumor necrosis factor is a pleiotropic pro-inflammatory cytokineand TNF receptor p55 (TNFRp55) mediates most of the TNFeffects. Pregnancy, either with or without signs of infection, showelevated levels of pro-inflammatory cytokines in maternal serumand fetal membranes when parturition is imminent. How thisinflammatory milliu could impact on the maternal synovialmembrane has not been investigated. Our objective was to study theconsequences of TNFRp55 deficiency on the interleukine(IL)-17and progesterone (Pg) levels in the joint of C57BL/6 wild type (WT)and TNFRp55-/- (KO) mice. IL-17 and Pg were determined byELISA and RIA, respectively. We found elevated levels of IL-17 atthe end of pregnancy in WT mice compared to diestrous stage.Moreover, IL-17 levels were lower in KO than WT mice at the end ofpregnancy (p<0.01). Interestingly, this cytokine exhibited acircadian profile in the joint of WT which was abolished in thedeficient mice. On the other hand, Pg also showed a circadianrhythm in both strains. The rhythm acrophase was advanced in theKO mice. Although, there was no difference in the levels of thishormone at the end of pregnancy, we found lower levels of Pg in KOmice at the diestrous (p<0.01). These results suggest that TNFRp55signaling plays a key role in the inflammatory process thatconstitutes normal-term labour as well as in its circadianmodulation.