Expression of pro-metalloproteinase-1 in human spondyloarthropathy fibroblast like synoviocytes by stimulation with Yersinia enterocolitica antigens

MARIA GABRIELA LACOSTE; ETHELINA CARGNELUTTI; RODRIGO VILLAREAL; MARIA SILVIA DI GENARO

Viña del Mar, Chile

Congreso; 9th Latin American Congress of Immunology, Immunochile 2009; 2009

Asociación Latinoamericana de Inmunología

Expression of pro-metaloproteinase-1 in human spondyloarthropathy fibroblast-like synoviocytes by stimulation with Yersinia enterocolitica antigens María Gabriela Lacoste, Ethelina Cargnelutti, Rodrigo Villareal, Héctor Tamashiro, María Silvia Di Genaro Divison of immunology, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Laboratory of Immunopathology, Multidisciplinary Institute of Biological Investigations-San Luis (IMIBIO-SL), CONICET, Argentina. Matrix metalloproteinases (MMP) degrade collagen and are induced during inflammatory responses. Spondyloarthropathies are a group of chronic disorders with articular tissue destruction. We investigated the effect of Yersinia antigens on MMP-1 (fibroblast collagenase) induction in human spondyloarthropathy fibroblast-like synoviocytes (FLS). FLS were isolated from synovial fluid of a patient with undifferentiated spondyloarthropathy, and used after passage 3 to 7. FLS were stimulated with heat kill Yersinia (HKY: 107 or 108 bacteria/ml). Yersinia lipopolyssacharide (LPS: 100 ng/ml) or zymozan (10 µg/ml), as a control, for 24, 48 and 72 h. MMP-1 activity and expression in culture supernatants were analyzed by casein zymography and Western blotting, respectively. The casein zymography yielded two bands (58 and 50 kDa) with proteolytic activity after HKY or LPS stimulation for 48 and 72 h. The induction of MMP-1 was confirmed by Western blot since a specif band corresponding to pro-MMP-1 was observed after HKY or LPS stimulation at the mentionated times. We concluded that Yersinia antigens induce pro-MMP-1 in spondyloarthropathy FLS and suggest that bacterial components in the joint could play a role in the pathogenesis and activation of spondyloarthropathies. Understanding the joint destructive process and identifying of MMPs are keys to the future treatment development.