EFFECT OF I.C.V. AGGREGATED β-AMYLOID (1-42) ON DAILY PATTERNS OF COGNITION-RELATED FACTORS IN RAT TEMPORAL CORTEX

CORIA LUCERO, C; GOLINI RS; ANZULOVICH AC; NAVIGATORE FONZO LS

Congreso; Tercera Reunión Conjunta de Sociedades de Biología de la República Argentina; 2015

Alzheimer?s disease (AD) is the most common form of dementia. Elevated levels of β-amyloid peptide (Aβ) in different areas of the Central Nervous System (CNS), including cortex, and progressive cognitive impairment, are characteristics of AD. Brain-derived neurotrophic factor (BDNF) and its high-affinity receptor, (TrkB), play a central role in the CNS by regulating synaptic plasticity and memory. TrkB and BDNF signaling are impaired in AD. Besides cognitive deficit, AD patients also show alterations in their circadian rhythms. Previously, we showed that aging Abolished Trkb circadian rhythms and phase shifted BDNF endogenous rhythms in thetemporal cortex. Continuing with that study, our objective was to investigate the effects of an i.c.v. injection of Aβ(1-42) peptide on the 24h rhythms of BDNF and TrkB expression, as well as on BMAL1 and Aβ protein levels in rat temporal cortex. Four-month-old males Holtzman rats were used in this study. Groups were defined as: control (CO) and Aβ-injected (Aβ). Rats were maintained under 12h-light:12h-dark conditions throughout the experimental period. Temporal cortex samples were isolated every 4 h during a 24h period. Transcript levels of BDNF and TrkB were determined by RT-PCR . Protein levels were analyzed by Western blots. We found that i.c.v. injection of Aβ(1-42) increased Aβ content and phase shifted daily variation of BDNF and TrkB expression in the rat temporal cortex, probably by altering the daily patterns of clock activator (BMAL1) as a result of Aβ accumulation. These results may constitute, at least in part, a molecular and cronobiological basis for deficits in temporal organization of temporal cortex-related cognitive functions in AD.