Effect of TNFRp55 deficiency on the Pg synthesis at the end of pregnancy.

DE LA VEGA M; RAGUSSA V; ANZULOVICH A; CASAIS M

Estancia Grande (San Luis)

Congreso; XXXII Reunion Anual de la Soc. de Biol. de Cuyo; 2014

Sociedad de Biología de Cuyo

An adequate rhythm of progesterone (P4) is crucial for determining the physiological duration of the estrous cycle and for achieving a successful pregnancy. Tumor necrosis factor is a pleiotropic cytokine and TNF receptor p55(TNFRp55) mediates most of the TNF effects. We previously showed that P4 levels and its synthetic enzyme (3β-HSD) exhibit a circadian rhythm in the ovary in diestrus. In this work we investigate the consequences of the lack of TNFRp55 in the P4 metabolism at the end of pregnancy, in C57BL/6 wild type (WT) and TNFRp55-/- (KO)mice. Animals were maintained on a 12-h light: 12-h dark cycle, at 24±2°C, with irradiated food and water available ad-libitum. Five days before the experiment mice were kept under constant darkness conditions. Ovaries were isolated every 6 h (24-48 h before delivery) during a 24h period. P4 and 3β-HSD protein levels were determined by RIA and Western blot, respectively. Similar to that we previously observed in diestrus, P4 levels arecircadian in the ovary of WT mice (p