Expression Of Retinoic Acid Receptors (RAR and RXR) and PPARs In Heart: Effect Of vitamin A deficiency

VEGA V.; ANZULOVICH A.C.; GIMÉNEZ MS; OLIVEROS L

Mar del Plata, Bs. As.

Congreso; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2007

SAIB

We have showed that vit A deficiency increases the activity of Carnitine Palmitoyltransferase I (CPT I), key enzyme in heart fatty acid -oxidación. Now, the effects of vitAon expressions of genes that are involved in the regulation of -oxidación are studied. Male Wistar rats at 21 d age were weaned onto either a vitAdeficient diet (-A) or the same diet with 8mg retinol/kg diet (control). They were fed for 3 months. Also, a -Agroup was refed for 15 days on control diet. Serum, liver and heart Vit A levels were measured by HPLC. The mRNAexpression of 9-cis-retinoic acid receptors (RXR and , and RAR ), peroxisome proliferator activated receptor and (PPAR and PPAR ), CPT I and Acetyl CoA carboxylase (ACC ), and also theACCactivity, were determined. Statistics was analyzed by ANOVA. In -A rats the mRNA expression of RAR , RXR , and RXR decreased (p< 0.01), while that of PPAR , PPAR and CPT I increased (p