PROTEOMIC ANALYSIS OF Amycolaptosis tucumanensis I

VILLEGAS LB, DELFINI CD

Puerto Madryn

Congreso; XLVI REUNION ANUAL SOCIEDAD ARGENTINA DE INVESTIGACION EN BIOQUIMICA Y BIOLOGIA MOLECULAR; 2010

SAIB

PROTEOMIC ANALYSIS OF Amycolaptosis tucumanensis IN RESPONSE TO CHROMIUMN AND COPPER STRESS 1 1 2 2 Villegas  LB , Delfini  CD ,  Schütze  E ,  Kothe  E ,  Amoroso M 1 Abate CM 1 2 PROIMI-CONICET,  Tucumán,  Argentina.  Friedrich  -  Schill University Jena, Germany. E-mail:  lbvilleg@hotmail.com Heavy  metal  pollution  is  one  the  most  serious  environmen problems. Some heavy metals play an important biological role trace  elements  but  are  toxic  at  higher  concentration Amycolatopsis  tucumanensis  was  isolated  from  contaminat sediments in Argentina. Its ability to remove Cu(II) and Cr(VI) h been  reported. The  aim  of  this  work  was  to  analize  the  differential  prote espression of A. tucumanensis under Cu(II) or/and Cr(VI) stress two-dimensional polyacrylamide gel elctrophoresis. Cells were incubated in minimal medium containing glucose a 10 ppm of Cu(II) or/and Cr(VI) during 72 h at 30ºC and 200 rp Cells grown in absence of heavy metals were used as control. T cells were chilled in liquid N2 and broken by physical techniqu The supernatants of  the cell  lysates was used as protein samp The proteome profiles were different, 18 spots were selected for  t identification  by  mass  spectrometry,  and  these  include dehydrogenase/reductase,  alkyldroperoxidase  Aph  D,  probab monoxigenase, RNA polimerase, cicloisomerase, some elongati factors,  transcripcional  regulator  and  several  proteins  wi unknown  function. Cu(II) or Cr(VI) resistance mechanisms are not known clearly, f this  reason  is very  important  to  study  the  functional  analysis these identified proteins and will allow to explain the tolerance heavy metals by  this bacterium.