Sexual dimorphism in the immunometabolic profile of Hofbauer cells

DANIEL PAPARINI; ESTEBAN GRASSO; FRANCO AGUILERA; BRENDA LARA; M. AGUSTINA ARSLANIAN; VICTORIA LELLA; VANESA HAUK; FÁTIMA MERECH; CLAUDIO SCHUSTER; MARÍA SOLEDAD GORI; CESAR MELLER; MARCELO MARTÍ; ROSANNA RAMHORST; DAIANA VOTA; CLAUDIA PÉREZ LEIRÓS

Congreso; Reunión Anual de La Sociedad Argentina de Inmunología; 2023

Aims: Hofbauer cells (HB) are the only foetal immune cell population within the stroma ofhealthy placenta. However, the functional properties of these cells are poorly described.HB are transcriptional and chromosomically similar to yolk sac macrophages. Here westudied the effect of sexual dimorphism in the immunometabolism of human placental HBat term.Methodology: HB cells were obtained from placental tissue by enzymatic digestion fromhealthy pregnant women at term along with autologous PBMCs. HB were culturedovernight and the phenotypic profile, efferocytosis, glucose uptake and lipid dropletsaccumulation were evaluated by flow cytometry and ELISA. We have started withbioinformatic analysis in public database to understand the differential imprinting ofplacentas with male or female newborns.Results: HB from female placenta (fHB) present higher production of antiinflammatorymarkers CD163, CD206 and CD39 accompanied by lesser secretion of IL-1B than HBfrom male placentas (mHB) (*P