Presence of cathelicidin LL37 and immunoendocrine-related compounds in THP1-derived macrophages infected with Mycobacterium tuberculosis exposed to cortisol and/or DHEA.

BONGIOVANNI BETTINA; MARTÍN LUEVANO, S; D'ATTILIO LUCIANO; DÍAZ ARIANA; FERNÁNDEZ, ROCÍO DEL VALLE; BAY MARÍA L; RIVAS-SANTIAGO BRUNO; BOTTASSO OSCAR

Buenos Aires

Congreso; Reunión Conjunta de las Sociedades Biomédicas; 2017

SAI

Macrophages (Mps) were found to be influenced in their anti-mycobacterial response since treatment with dehydroepiandrosterone (DHEA) of Mps infected with Mycobacterium tuberculosis (Mtb) led to a reduction in intracellular bacterial load and increased autophagy. On the other hand, the Mp effector response in the tuberculosis infection is highly related to the production of antimicrobial peptides such as cathelicidin LL37, whose production was found increased during autophagy, as well. Given this background, THP-1-derived Mps were infected with Mtb strain H37Rv (MOI 5:1) during 3 h in presence or absence of cortisol (Gc, 1 and 0.1μM) and/or DHEA (1; 0.1 and 0.01μM). Upon washing, cells were cultured for 24 h in the same media with or without hormones and the expression of LL37, glucocorticoid receptors -alpha and beta- (GRα and GRβ), enzymes regulating cortisol availability (11-beta-hydroxysteroid dehydrogenase types 1 -11βHSD1- and 2), the pro-apoptotic protein caspase 3, the inflammasome-related protein 3 (NLRP3) were analyzed by qRT-PCR, and IL1β levels by ELISA.NLRP3 and caspase 3 showed an increase in all infected cultures (p