Evaluation of different immuno-endocrine factors influencing migratory activity of t cells in individuals with chronic chagasic myocarditis.

GONZÁLEZ FLORENCIA; PACINI ANTONELA; BERBERT LUIS; VILLAR SILVINA ; VIGLIANO CARLOS; D'ATTILIO LUCIANO; GALLUCCI, GEORGINA; BOTTASSO OSCAR; SAVINO, WILSON

CABA

Congreso; Reunión Conjunta SAIC. SAI. AAFE. NANOMED-AR.; 2021

SAIC. SAI. AAFE. NANOMED-AR.

T cell-inflammatory infiltrates are evident in the heart of individuals with chronic chagasic myocarditis (CCM). T cell trafficking into the hearts of CCM patients may be modulated by systemic inflammation and by in situ expression of chemotactic or haptotactic factors. In addition, systemic glucocorticoids (GC) might modulate T cell activation and migratory response. Our objective was to evaluate immuno-endocrine factors that can differentially influence the migratory capacity of T cells in individuals with CCM. Thus, we evaluated in the heart tissue from CCM and seronegative (Co) individuals subject tocardiac transplant (n=3/group), inflammatory infiltrates and fibrosis (by H&E and trichome stain) and the expression of chemotactic/haptotactic factors as fibronectin (FN), CXCL12 and TNFα (by immunofluorescence). In addition, we determined in PBMCs from CCM andCo (n=20/group) the expression of HLADR as an activation marker and VLA4 (FN receptor) by cytometry and TNFR1/2 by RTqPCR. Moreover, GC sensibility was estimated by GC receptors (GRα as functional receptor and GRβ as inhibitor receptor) and 11β-HSD1 (catalyzes the conversion of GC from inactive to active form) expression by RTqPCR. CCM tissue sections exhibited obvious infiltrates and fibrosis and an increased immunoreactivity for FN, CXCL12 and TNFα (p