MELANOMACRÓFAGOS HEPÁTICOS Y ACTIVIDAD DE LA ENZIMA GLUTATION S-TRANSFERASA EN Leptodactylus chaquensis (ANURA, LEPTODACTYLIDAE) COMO BIOMARCADORES DE ESTRÉS OXIDATIVO POR LA EXPOSICIÓN A CLORPIRIFOS

HUESPE IVAN; CABAGNA-ZENKLUSEN MARIANA; CURI LUCILA MARILÉN; PELTZER PAOLA; ATTADEMO MAXIMILIANO; VILLAFAÑE NOELIA ; LAJMANOVICH RAFAEL

Acta Biológica Colombiana

Uniersidad Nacional de Colombia

Lugar: Bogotá; Año: 2017 vol. 22 p. 234 - 237

0120-548X

We quantified and compared the hepatic melanomacrophage (MM) and glutathione S-transferase (GST) enzyme activity (two oxidative stress biomarkers) in the liver of Leptodatylus chaquensis adults (Anura, Leptodactylidae) collected in a rice field (CA) in San Javier department, Santa Fe (Argentina), seven days after the application of chlorpyrifos and in a reference site (SR). The histological analysis revealed a significant amount (p = 0.028) and area occupied by MM (p = 0.017) in livers of CA compared to SR. Furthermore, significant inhibition of GST activity was recorded in the CA frogs compared to the SR (p = 0.030). The histopathological and enzymatic effects provide evidence of ecotoxicological risk for anurans in the rice field with CPF application.