Molecular determination of B-glucosidase activity

HÉCTOR A. CRISTÓBAL; CARLOS M. ABATE

San Carlos de Bariloche, Argentina

Congreso; XXXIX Reunión Anual de Sociedad Argentina de Investigación Bioquímica y Bilogia Molecular (SAIB); 2003

SAIB

Psychrophiles are extremophiles microorganism that have the ability to grow and colonize environments where the temperature is close to 0 ºC. Cold-adapted microorganisms are interesting for manufacturers who need cold-storage room temperatures enzymes for food processors and cold-wash laundry detergents. The ¥â-glucosidase is an enzyme able to hidrolize cellobiose and glucosides related. The main of this work was to isolate and characterize of ¥â-glucosidases activity marine bacteria present in sea water and bentonic organisms samples. The microorganisms were isolated from the South of Argentina sea water. Samples were taken from different sites of the Beagle Channel (55¨ºS; 67¨ºW) and intestinal content from bentonic organisms. They were cultivated in a solid medium added of cellobiose and incubated at 4 ºC and 15 ºC in shaking. In order to determine the presence of b-glucosidase gene PCR reactions were carried out. Microorganisms were selected by the growth on plate agar containing cellobiose and by yellow color reaction over the colony using an analogous PNPG. b-Glucosidase activity was found in 32 colonies of the 48 isolates. ¥â-glucosidase positive. According with the ITS profiles, the isolates were divided in 6 different groups. Microorganism of one group of its was sequencing the 1,500 bp of the 16S rDNA and of ¥â -glucosidase gene, to determine their association with the groups and the class of bacterias. The obtained results are industrial interest because can provide new cold active products for biotechnological use.