INVESTIGADORES
CORVI maria Martha
artículos
Título:
Rabbit antibodies against Toxoplasma Hsp20 are able to reduce parasite invasion and
Autor/es:
COCERES, VM; ALONSO, AM; ALOMAR, ML; CORVI, MM
Revista:
EXPERIMENTAL PARASITOLOGY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 132 p. 274 - 281
ISSN:
0014-4894
Resumen:
Toxoplasma gondii Hsp20 is a pellicle-associated functional chaperone
whose biological role is still unknown. Hsp20 is present in different apicomplexan
parasites, showing a high degree of conservation across the phylum, with
whose biological role is still unknown. Hsp20 is present in different apicomplexan
parasites, showing a high degree of conservation across the phylum, with
whose biological role is still unknown. Hsp20 is present in different apicomplexan
parasites, showing a high degree of conservation across the phylum, with
Hsp20 is a pellicle-associated functional chaperone
whose biological role is still unknown. Hsp20 is present in different apicomplexan
parasites, showing a high degree of conservation across the phylum, with
Neospora caninum Hsp20 presenting an 82% identity to that of T. gondii. Hence
rabbit anti-T. gondii Hsp20 serum was able to recognize the N. caninum
rabbit anti-T. gondii Hsp20 serum was able to recognize the N. caninum
rabbit anti-T. gondii Hsp20 serum was able to recognize the N. caninum
Hsp20 presenting an 82% identity to that of T. gondii. Hence
rabbit anti-T. gondii Hsp20 serum was able to recognize the N. caninumT. gondii Hsp20 serum was able to recognize the N. caninum
counterpart. Interestingly, both N. caninum and T. gondii Hsp20 localized to the
inner membrane complex and to the plasma membrane. Incubation of T. gondii
inner membrane complex and to the plasma membrane. Incubation of T. gondii
inner membrane complex and to the plasma membrane. Incubation of T. gondii
N. caninum and T. gondii Hsp20 localized to the
inner membrane complex and to the plasma membrane. Incubation of T. gondiiT. gondii
and N. caninum tachyzoites with an anti-TgHsp20 serum reduced parasite invasion
at rates of 57,23% and 54.7% respectively. This anti-serum also reduced T. gondii
at rates of 57,23% and 54.7% respectively. This anti-serum also reduced T. gondii
at rates of 57,23% and 54.7% respectively. This anti-serum also reduced T. gondii
N. caninum tachyzoites with an anti-TgHsp20 serum reduced parasite invasion
at rates of 57,23% and 54.7% respectively. This anti-serum also reduced T. gondiiT. gondii
gliding 48.7%. Together, all this data support a role for Hsp20 in parasite invasion
and gliding motility.