INVESTIGADORES
COHEN ana Carmen
congresos y reuniones científicas
Título:
Characterization and antioxidant potential of carotenoids produced by Bacillus licheniformis Rt4M10.
Autor/es:
DICHIARA E,; COHEN A C,; JOFRÉ V,; BOTTINI R,; PICCOLI P N
Lugar:
Mendoza
Reunión:
Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Carotenoids are the most widespread naturally occurring yellow, orange and red pigments. These can be grouped into two major classes: carotenes (hydrocarbons that can be cyclized at one or both ends of the molecule), and their oxygenated derivatives called xanthophylls. Several species of bacteria accumulate high concentrations of carotenoids. They are of interest due to theirantioxidant, UV protecting and natural food colorant properties. In a previous study B. licheniformis Rt4M10 (Rt4M10) was isolated from roots of Vitis vinifera L. (cv. Malbec). The aim of this study was to identify and quantify carotenoids produced by Rt4M10 and its biological role in vivo. Rt4M10 was cultivated aerobically in orbital shaker. Cells were centrifuged and the upper layer was discarded and residual cells were extracted with methanol. Suspensions were sonicated and centrifuged. After that, the clear coloured organics phases were transferred into a clear tube. Finally, the carotenoids levels in the supernatant were quantified through HPLC. To evaluate sensitivity to hydrogen peroxide (H2O2), the cells were cultivated until the stationary phase. Accurate quantities of H2O2 solution were added to the cell suspension, it was incubated with H2O2 in the dark and it was plated. Colonies were counted after 4-5 days to evaluate antioxidant capacity. Lycopene, β-carotene, ɡ-carotene, violaxanthin, criptoxantine and zeaxanthin were identified and quantified by HPLC. These carotenoids demonstrated antioxidant activity in vivo.