Azospirrillum brasiliense promueve la síntesis de terpeno ciclasas bajo condiciones de estrés salino

COHEN A.; GIL M; PICCOLI P

Huerta Grande, Córdoba

Jornada; I Reunión Conjunta de Sociedades de Biología de la República Argentina, XVI Jornadas Científicas de la SBC, IX Jornadas de la SAB y XXV Reunión Anual de la SBCuyo con la participación de la SBRosario y de la Asociación de Biología de Tucumán; 2007

Sociedad de Biología de Córdoba, Cuyo, Rosario y Tucumán

Azospirillum brasilense PROMOTES TERPENE CYCLASE ACTIVITY UNDER STREsS BY NaCl   Ana C. Cohen, Mariana Gil and Patricia N. Piccoli Cát. Quím. Org. y Biológica, Fac. Cs. Agrarias-CONICET, Univ. Nac. Cuyo, Alte. Brown 500, M5528AHB, Chacras de Coria. Email: ppiccoli@fca.uncu.edu.ar   Azospirilllum brasilense are plant growth promoter bacteria (PGPB) that improve yield in cereals and others species. The beneficial effects of PGPB are attributed to production of terpenic phytohormones like ABA. ABA production by A. brasilense in chemically-defined media was doubled when 100 mM  NaCl was added to the medium. In stressed plants FPP is cyclized by sesquiterpene-cyclases (TPS) to produce phytoalexins. The aim of this work was to evaluate sesquiterpene-cyclase activity in A. brasilense cultures with NaCl. Bacteria were cultured in NFB medium fed with 3H-FPP.  Addition of 100 mM NaCL enhanced 40-fold the proportion of radioactivity related with terpene cyclase activity. These results suggest that A. brasiliense elicitate synthesis of sesquiterpene phytoalexin-like compounds under NaCL stress conditions. Area temática: Biología Celular y Molecular                                                                                               Subárea: Microbiología