Lindane removal from contaminated soil by Streptomyces M7

BENIMELI CS; FUENTES MS; CHAILE AP; ABATE CM; AMOROSO MJ

Pinamar- Buenos Aires- República Argentina

Congreso; X Congreso PABMB- XLI Reunión Anual SAIB- XX Reunión Anual SAN; 2005

Asociación Panamericana de Bioquímica y Biología Molecular (PABMB)- Sociedad Argentina de Investigación en Bioquímica y biología Molecular (SAIB)- Sociedad Argentina de Neuroquímica (SAN)

Lindane, that has been extensively used worldwide for the control of agricultural and medical pests, is a common pollutant found ubiquitous in the biosphere. The use of indigenous actinomycetes for bioremediation of lindane contaminated soils is an attractive approach, since these microorganisms are already adapted to the habitat. The aim of this work was to investigate the ability of Streptomyces M7 to remove lindane from contaminated soils. Sterile soils contaminated with lindane 100 mg Kg-1 were inoculated with Streptomyces M7 0.5 g/Kg and incubated by 28 days. Bacterial growth was determined by UFC/g and residual lindane by GC; previously extractions with acetone and hexane were made. Two weeks after inoculation of Streptomyces M7 into lindane-amended autoclaved soil, the strain removed 25% of the pesticide. The microbial population increased from 6.7.105 (0 day) to 3.7.109 (14 days). Similar results were observed in the bacterial growth in control non contaminated soils.