Selection of an actinobacteria mixed culture for chlordane remediation. Pesticide effects on microbial morphology and bioemulsifier production

FUENTES MS; COLIN VL; AMOROSO MJ; BENIMELI CS

JOURNAL OF BASIC MICROBIOLOGY

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2016

0233-111X

Chlordane bioremediation using actinobacteria mixed culture is an attractive clean-up technique.Their ability to produce bioemulsifiers could increase the bioavailability of this pesticide. In order toselect a defined actinobacteria mixed culture for chlordane remediation, compatibility assays wereperformed among sixStreptomyces strains. The strains did not show growth inhibition, and they wereassayed for chlordane removal, either as pure or asmixed cultures. In pure cultures, all of the strainsshowed specific dechlorination activity (1.42?24.20 EUmg1) and chlordane removal abilities(91.3?95.5%). The specific dechlorination activity was mainly improved with cultures of three orfourmicroorganisms. Themixed culture consisting of Streptomyces sp.A2-A5-A13was selected. Theirability to produce bioemulsifiers in the presence of glucose or chlordane was tested, but nosignificant differences were observed (p > 0.05). However, the stability of the emulsions formed waslinked to the carbon source used. Only in chlordane presence the emulsions retained 100% of theirinitial height. Finally, the selected consortium showed a high degree of sporulation in the pesticidepresence. This is the first study on the effects that chlordane exerts on microbe morphology andemulsifier production for a defined mixed culture of Streptomyces with ability to remediate thepesticide.