Isolation of four aquatic streptomycetes strains capable of growth on organochlorine pesticides

C.S. BENIMELI; M.J. AMOROSO; A.P. CHAILE; G.R. CASTRO

BIORESOURCE TECHNOLOGY

ELSEVIER LTD.

Lugar: Oxford, UK; Año: 2003 vol. 89 p. 133 - 138

0960-8524

Ninety-three wild-type isolates identified as actinomycetes were tested against 11 organochlorine pesticides (OPs): aldrin, chlordane, DDD, DDE, DDT, dieldrin, heptachlor, and heptachlor epoxides, lindane, and methoxychlor. Qualitative screening agar assays displayed 62?78% tolerance of strains to OPs. Four strains designed M4, M7, M9 and M15 were selected based on multi-OPtolerance, and identified as members of the streptomycetes group. Different growth profiles were observed in cultures of the four selected streptomycetes cultured in synthetic medium containing 5?50 lg l1 aldrin or chlordane or lindane. Increase of aldrin removal by the selected microorganisms was concomitant with the 4.8?36.0 lg l1 pesticide concentration range. After 72 h of streptomycete M7 growth in synthetic medium containing 48.0 lg l1 aldrin, the remaining OPconcentration in the supernatant was approximately 10% of the initial concentration. Also, in stationary growth phase less than 2.5 lg l1 aldrin residual concentration was detected in the medium.