Applied of actinobacteria consortia-based bioremediation to restore co-contaminated systems

ANTEZANA, PABLO E.; COLIN, VERÓNICA L.; BOURGUIGNON, NATALIA; BENIMELI, CLAUDIA S.; FUENTES, MARÍA S.

RESEARCH IN MICROBIOLOGY

ELSEVIER SCIENCE BV

Año: 2023

0923-2508

The global industrialization and natural resources extraction have left cocktails of pollutants in the environment. Thus, this work focuses on the development of a defined actinobacteria consortium, able to restore systems co-contaminated with pollutants occurring in Argentinian environments. For that, five actinobacteria were tested in solid medium to determine antagonism among them and its tolerance against lindane (LIN), Reactive Black B-V (RBV), phenanthrene (Ph) and Cr(VI). Bacteria shown absence of antagonism, and most of them tolerated the individual pollutants and mixtures tested, except Micromonospora sp. A10. Thus, a quadruple consortium constituted by Streptomyces sp. A5, M7, MC1 and Amycolatopsis tucumanensis DSM 45259T was tested in individually contaminated liquid systems. The best growth was observed on RBV presence, and the lowest on Cr(VI). Removals detected were 83.3%, 65.0% and 52.4% for Ph, RBV and LIN, respectively, with absence of Cr(VI) dissipation. In consequence, the consortium performance was tested against the organic mixture, observing a microbial growth similar to the biotic control, and a LIN removal increase (61.2%). Moreover, the four actinobacteria of the consortium survived to the mixture bioremediation process. These results demonstrate the potential of the defined actinobacteria consortium as a tool to restore environments co-contaminated with organic pollutants.