INVESTIGADORES
ROMERO cintia mariana
artículos
Título:
Direct DNA amplification from FALL ARMYWORM (LEPIDOPTERA: NOCTUIDAE) samples
Autor/es:
FLAVIA LOTO1, CINTIA M. ROMERO, MARIO D. BAIGORÍ1, 2 AND LICIA M. PERA1
Revista:
FLORIDA ENTOMOLOGIST
Editorial:
FLORIDA ENTOMOLOGICAL SOC
Referencias:
Lugar: Nueva York; Año: 2013 vol. 96 p. 1618 - 1620
ISSN:
0015-4040
Resumen:
The direct amplification by PCR of the DNA in tissue samples of eggs and neonate larvae of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) was accomplished, and it is a new alternative for DNA amplification from fall armyworm samples. This method is simple, fast, economic, and accelerates studies on this polyphagous pest. Key Words: DNA extraction, DNA purification, PCR amplification, sequencing, strain identification