INVESTIGADORES
ROMERO cintia mariana
artículos
Título:
Design of an Immobilized Biohybrid Catalyst by Adsorption Interactions onto Magnetic Srebrodolskite Nanoparticles
Autor/es:
MORALES, ANDRÉS H.; HERO, JOHAN S.; NAVARRO, MARÍA C.; FARFÁN, ELSA M.; MARTÍNEZ, MARÍA A.; LAMAS, DANIELA L.; GÓMEZ, MARÍA I.; ROMERO, CINTIA M.
Revista:
ChemistrySelect
Editorial:
Wiley
Referencias:
Año: 2019 vol. 4 p. 11024 - 11033
ISSN:
2365-6549
Resumen:
In this work,anovelnanobiocatalyst(L?Mcn4@Ca2Fe2O5) wassynthesizedfromBacilluslipase onsrebrodolskitenanoparticles.Bacillussp. MCn4culture mediawasoptimizedreaching themaximumlipolytic activityusingpeptone7.5g/Landsucrose5g/LandCa2Fe2O5, wassynthesizedandcharacterized(size of100?150nm).L?Mcn4@Ca2Fe2O5 wasobtainedbyadsorption(12U/mgoxide) and successfully characterized by differenttechniques. Accordingto the bestimmobilization conditions,pH4and100Mmionic strength, anadsorptionmodelwithtwo stageswasproposed:initial interfacial activationfollowedby multi-point electrostatic interactions. Stability studiesshowed anenhancementin its performancebetween50and70°Candagainstethanolcomparedwithfree enzymeandthethermal decompositionprocesswassofter whenlipase wasimmobilized. The biocatalyst hydrolyzed fish oil showingenrichmentin the PUFAscontentwithanincrease onDHAof1.2 fold showing a great potential for its use in the foodindustry.
