Draft Genome Sequence of Achromobacter sp. Strain AR476-2, Isolated from a Cellulolytic Consortium

KURTH, DANIEL; ROMERO CINTIA M; FERNANDEZ PABLO M; FERRERO MARCELA A.; MARTINEZ M. ALEJANDRA

Genome Announcements

The American Society for Microbiology

Año: 2016 vol. 4 p. 1 - 2

Achromobacter sp. AR476-2 is a Gram-negative isolated from acellulolytic bacterial consortia selected from the guts of Diatraeasaccharalis larvae (1). Isolates belonging to this genus weredescribed as opportunistic pathogens (2) and the potential forenvironmental bioremediation applications has been recently targeted(3). The genomic analysis showed that AR476-2 strain isclosely related to A. piechaudii strain HLE (4) and to A. xylosoxidansA8 (5).The genome sequencing of the strain AR476-2 was performedusing two lanes in an Illumina HiSeq 1500, producing 20,525,460paired-end reads (2 100 bp) with a median sequencing coverageof 438. Quality filtering and trimming was performed with Perlscript (6). A complete quality control analysis with Fast QC wasalso performed on the resulting reads (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Paired-end reads were assembledinto 61 scaffolds with the A5 assembler (7). Automatic annotationusing the RAST (8) web server was carried out to for initialcharacterization and the submitted genome was annotated by theNCBI Prokaryotic Genome Annotation Pipeline (http://www.ncbi.nlm.nih.gov/genome/annotation_prok/).