Relationships between reactive oxygen species and growing variables of Pistum sativum L., under different soil moisture levels

MICHEL ANALIA; APPELHAS, STEFANÍA C.; SEQUIN CHRISTIAN J.; BIANCHI MARIANA; TROSSERO JULIA A.; CAVIGLIA OCTAVIO P.

Congreso; XXXIV Argentinian Meeting of Plant Physiology RAFV Conference 2023; 2023

Sociedad Argentina de Fisiología Vegetal

Low aeration capacity of soils may impact biological nitrogen fixation, especiallyin waterlogging-sensitive species like Pisum sativum L. It is well recognized thatroot nodules exhibit intense biochemical activity and that the production ofreactive oxygen species (ROS) is influenced by this activity. However, little isknown about how soil low aeration capacity affects nodulation and how itimpacts ROS production in other plant organs, such as stipules. The aims ofthis study were: 1) to evaluate the effect of different levels of soil aeration onnodulation, and 2) to assess the effect of nodulation on ROS production andantioxidant enzyme activity throughout the stipules of P. sativum. A greenhousestudy was conducted to evaluate P. sativum plants growing and ROS productionunder two different soil moisture levels, 100% and 60% of soil field capacity(100% FC and 60% FC, respectively). After 30 days of pot irrigation, numberand weight of nodules, and aerial and root biomass were measured. ROSmeasurements included the determination of hydrogen peroxide (H2O2)concentration and superoxide (O2-) concentration in stipules. Ascorbate peroxidase (ASP) and catalase (CAT) enzymatic activity were also measured.Number of nodules, aerial and root biomass were significantly higher for 100%FC compared to 60% FC treatment. Soil moisture levels did not affected O2-concentration nor enzymatic activities. However, the treatment of 100%FC,significantly increased H2O2 concentration in stipules (p