Phenolic compounds and biological potential of hidroetanolic extracts of Schinus areira, Aphyllocladus spartioides and their binary mixtures

LILIANA SOLEDAD CELAYA; LUIS RODRIGUEZ SILVA; CARMEN INES VITURRO

Cordoba

Congreso; V Congreso Internacional de Ciencia y Tecnología de los Alimentos Córdoba CICyTAC 2014; 2014

Ministerio de Ciencia y Tecnología - Gobierno de la Provincia de Cordoba - UNC

 The aims of this work were to improve the knowledge on phenolic composition and evaluate the in vitro biological activity of Schinus areira (leaves) and Aphyllocladus spartioides (leaves) hidroetanolic extracts and their mixtures, for further potential exploitation. Following these purposes, crude extracts of molle and tojra tola leaves collected from Jujuy (Argentina) were obtained by sonication with ethanol:water (70:30). Extracts alone and their mixtures were tested by invitro assays in order to evaluate their antioxidant and α-glucosidase activities; the presence of synergic, additive or antagonic effects were estimated by FICindex; additionally, phenolic composition was performed by HPLC/DAD. Mixture with 50% (w/w) of each extract showed the highest inhibition for DPPH radical. A concentration-dependent activity was noticed against the tested radicals; synergic effects were observed against NO, not being found for O2− radical. Additionally, α-glucosidase inhibitory activity was also evaluated, a concentration-dependent activity for tested extracts and synergistic effect for the mixture with 50% of both extracts were observed. The main phenolic compounds found in S. areira leaves extracts were quercetin derivatives and caffeoylquinic acids; isorhamnetin derivatives were found in higher amounts in A. spartoides. The 50% mixture revealed a rich combination of these phenolic compounds; S. areira leaves extract not only enhances the antioxidant efficiency, but also the antidiabetic effect of A.spartioidesleaves extract.