Chemical composition and biological prospecting of essential oil and extracts of Aphyllocladus spartioides growing in Northwest Argentina

LILIANA CELAYA; LUIS RODRIGUEZ SILVA; CARMEN INES VITURRO

Chemistry and Biodiversity

WILEY-V C H VERLAG GMBH

Año: 2017 vol. 14 p. 1 - 10

1612-1872

Aphyllocladus spartioides Weddell is a native and aromatic herb used intraditional medicine, however it is still poorly investigated. In this work, the volatileprofile of A. spartioides growing in Hornillos-Northwest Argentina was determined byGC-MS/FID and the phenolic compounds of hydroethanolic and decoction extractswere analyzed by HPLC-DAD. The antibacterial potential, antioxidant activity and α-glucosidase inhibition activity were checked by in vitro assays. The volatile profileallowed the identification of 68 compounds, being α-pinene and cadinene the mainones. Eighteen phenolics were identified, isorhamnetin derivatives and differentphenolic acids were found in higher amounts, mainly in the hydroethanolic extract. Aconcentration-dependent activity was noticed against DPPH?, O2??, ?NO and α-glucosidase, these activities being reported for the first time. Hydroethanolic extract wasmost active against DPPH?, ?NO and α-glucosidase (IC50 = 79, 206 and 181 μg/mL).Decoction extract proved to be better against O2?? (IC50 = 20 μg/mL). Regarding theantibacterial activity, hydroethanolic extract was more active compared with decoctionand essential oil. MICs of 0.3-0.6 mg/mL were obtained against Staphylococcus aureus,Bacillus cereus and Micrococcus luteus. Results suggest that the extracts of A.spartioides from Northwest Argentina may be interesting to use as a source of naturalantioxidants/antimicrobials for pharmaceutical incorporations or food supplementation.