Metabolic and biological prospecting of Aphyllocladus spartioides

LUIS R SILVA; LILIANA S CELAYA; BLANCA SILVA; CARMEN I VITURRO

Budapest

Simposio; 63rd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA2015); 2015

Aphyllocladus spartioides known as tojra-tola is a native shrub distributed insouthern Bolivia and the northwest of Argentina, being used medicinally byindigenous people for the treatment of digestive infections and to treatrheumatic diseases. In order to improve the knowledge on its metabolite profileand biological properties, two different leaf extracts (hydroethanolic anddecoction) were studied. 18 phenolic compounds were determined by HPLC-DAD. Thephenolic content of the hydroethanolic extract was higher, isorhamnetin-3-O-glucosidebeing the main constituent. 8 organic acids were identified by HPLC-UV, malic acidwas the compound present in the highest amount in both extracts. The chemicalprofile of essential oil of A. spartioides leaves was also characterizedby HS-SPME/GC-FID, and 67 volatiles were identified. Cadinene was the mostabundant constituent. The inhibitory effects against cholinesterases, theantioxidant potential (DPPH*, O2*- and *NO) and the antibacterial activity were checkedby in vitro assays. Decoction was the most active against the first two radicals(IC50=79 and 28 ug/mL, respectively). Thet hydroethanolic extract proved to bethe most active on nitric oxide (IC50=206), acetylcholinesterase (IC50=926), butyrylcholinesterase(IC50=917) and αglucosidase (IC50=181). Additionally, antibacterial activitywas also investigated here against a set of pathogenic bacteria. The bioactivitiesobserved may be due, at least partially, to the presence of organic andphenolic acids, flavonoids and volatiles determined in this work. Furthermore, A.spartioides can be used in food industry as food additive, or can beapplied in cosmetic and pharmaceutical industries providing protections againstseveral diseases