CONICET | Buscador de Institutos y Recursos Humanos

The aim of this study was to identify Aspergillus sp. LBM 134, isolated in Misiones, Argentina.The identification was performed by the online service Polyphasic Identification of the Institute ofFungal Biodiversity Westerdijk (CBS) and included molecular, macromorphological andmicromorphological analysis, as well as acid production tests. Macromorphological characteristicswere observed by incubating the fungus on Czapek Yeast Extract Agar (CYA), Czapek YeastExtract NaCl (CYAS), Malt Extract Agar (MEA) and Creatine Sucrose Agar (CREA) at differenttemperatures for 7 d. Diameters and colors of mycelial colonies were observed and measured.Production of cyclopiazonic acid and other alkaloids that react to Ehrlich were examined using thefilter paper method. Micromorphological characteristics were observed by optic microscope usingthe lactophenol cotton blue method and were analyzed with ImageJ 1.47v program. DNA wasextracted from the mycelium grown on Yeast Extract Sucrose (YES) at 25 °C for 3 d. Molecularmarkers ITS, Bt, CAM, D1/D2 and Tef were amplified with ITS1-ITS4, Bt2a-Bt2b, CMD5-CMD6and EF1S-Tef1R universal primers respectively; later they were sequenced. DNA sequences wereedited with Chromas v2.6.5 and BioEdit Sequence Alignment Editor v7.0.5. LBM 134 has biseriateconidiophores, large and hyaline stipe (1000 - 1500 µm), light brown sclerotia and black globose tosub globose conidia (3 x 2.6 µm) with finely echinulate ornamentation. Also, produced acid onCREA media and did not produce alkaloids. All this information gathered to the polyphasicidentification resolved that LBM 134 is A. niger. Polyphasic identification is a safe, accurate, andsensible method for the identification and differentiation of species of the genus Aspergillus.

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