INVESTIGADORES
CASTRILLO Maria lorena
congresos y reuniones científicas
Título:
PREDICTION OF ACTIVATORS OF THE PROTEASE PR1 GENE INVOLVED IN BEAUVERIA BASSIANA BIOCONTROL
Autor/es:
BICH, GA; CASTRILLO, ML; VILLALBA, LL; ZAPATA, PD
Lugar:
Modalidad virtual
Reunión:
Congreso; 1st Latin American Congress of Women in Bioinformatics and Data Science 2020; 2020
Resumen:
Beauveria bassiana is a cosmopolitan, soil-born, and the most important entomopathogenic fungus used in different agricultural biological products. B. bassiana can secrete different enzymes, such as proteases, chitinases, among others, involved in biocontrol. Extracellular proteases have been shown to be virulent factors in fungal pathogenicity toward insects. Promoter sequences and transcription factors (that bind to them), are major regulatory elements of gene expression. Their recognition by computer algorithms is a new and encouraging strategy for understanding gene expression patterns. This study aimed to verify in silico the genetic variability of the subtilisin-like PR1A protease gene in B. bassiana isolates and predict possible binding sites for regulators of this gene which results can improve our knowledge on B. bassiana enzyme secretion and biotechnological use in biocontrol. Genetic and genomic sequences of PR1A proteases genes involved in B. bassiana biocontrol were retrieved from NCBI database and curated. The sequences were analyzed for possible binding sites for regulators of enzyme production located until 1 Kb upstream. UNIPROT and JASPAR CORE databases were used for the analyses. This procedure allowed us to obtain and predict the regulation region of the PR1A protease gene of B. bassiana of 1558 bp, and obtain regions with potential use in the modulation of the secretion of PR1A proteases involved in biocontrol. It was possible to predict a total of 80 putative binding sites for transcription factors, and even some of them were previously reported as activators of genes in other biological systems. There were found some interesting binding sites for transcription factor for eukaryotes like CAT8, which was reported as a positive regulator of transcription by RNA polymerase II. Or MSN2 which acts as a positive transcriptional factor that acts as a component of the stress responsive system (heat, pH, oxidative, osmotic, etc.).