THE GENOME OF TRICHODERMA KONINGIOPSIS CARRIES 19 GENES ENCODING ENZYMES INVOLVED IN CELLULOSE DEGRADATION

CASTRILLO, ML; BICH, GA; ZAPATA, PD; SAPARRAT, MCN; VILLALBA, LL

Modalidad virtual

Congreso; 1st Latin American Congress of Women in Bioinformatics and Data Science 2020; 2020

Cellulose is the most abundant polysaccharide in nature. Three main types of cellulase are required for cellulose hydrolysis to glucose: endoglucanases (EGs), β-glucosidases (BGLs) and cellobiohydrolases (CBHs). The Trichoderma koningiopsis POS7 isolate secretes high levels of cellulolytic-active enzymes and is therefore a promising strain for use in biotechnological applications. However, the T. koningiopsis genes encoding enzymes involved in cellulose hydrolysis have not been well explored. The present work analyses the genome of this fungus for identifying putative genes related to cellulase degradation. Genomic DNA library construction and draft genome sequencing were performed by Macrogen using the Illumina MiSeq system. These sequences were assembled de novo using the SPAdes software. To predict genes we used two ab initio gene predictor, AUGUSTUS and FgenesH. A database was made with the nucleotide sequences of genes that code for cellulolytic enzymes (EGs, BGLs and CBHs) in other species of Trichoderma and related fungi, as well as also from their available deduced amino acid sequences, which were compared with the annotation files generated by AUGUSTUS and FgenesH. These sequences were blasted in the NCBI database in order to determine identity (ie, regions containing sequences of the genes encoding cellulases). In addition, Integrative Genomics Viewer and Geneious software were used to determine the complete structural regions of each gene of interest. The cellulolytic enzymes genes in the genome of T. koningiopsis POS7 were scattered in 15 assembled scaffolds. We predicted and annotated 19 cellulolytic enzymes genes: seven EGs, ten BGLs and two CBHs. The cellulase genes were located mainly in the minus strand except for four genes encoding BGLs enzymes. The genes encoding CBHs enzymes were located in the same scaffold of two EGs enzymes genes. This information will be key to select a candidate gene to study the in vitro cellulose degradation.