CASTRILLO Maria lorena
congresos y reuniones científicas
MOLECULAR IDENTIFICATION AND PHYLOGENY OF TWO ENDOPHYTIC PLANT GROWTH-PROMOTING SPORE-FORMING BACTERIA ISOLATED FROM YERBA MATE (ILEX PARAGUARIENSIS ST. HIL.)
CORTESE, IJ; CASTRILLO, ML; ONETTO,A; GORTARI, F; MALLOZZI, G; ZAPATA, PD; LACZESKI, ME
FOZ DO IGUAZU
Congreso; INTERNATIONAL CONGRESS OF GENETICS; 2018
Currently, the use of beneficial microorganisms that enhance the growth of crops and act as soil recovery agents proves to be a promising alternative towards sustainable agriculture. Our working group isolated two endophytic bacteria from Ilex paraguariensis St. Hill. (yerba mate) coded as 12RS3 and T5S-T4. These bacteria showed properties to promote plant growth and biofertilize yerba mate nurseries with good results. The objective of the present work was to molecularly identify two endophytic plant growth-promoting spore-forming bacteria isolated from yerba mate plants. Genomic DNA was extracted from the bacteria incubated in liquid cultures at 30°C for 24 h. For the molecular identification, the 16S rDNA region was amplified and sequenced using the universal primers EUB9_27 and EUB1542. A consensus sequence was generated by Geneious 3.6.1 program, and the obtained information was compared with the deposited information in the National Center for Biotechnology Information database and the EzTaxon-e database (EZBioCloud) using its online identification service. The sequences with the highest percentage of similarity were selected and analyzed by the Neighbor-Joining method using a Bootstrap test with 1000 replications using the Mega 6.06 software. The analysis of identity and similarity and the construction of monophyletic clades allowed us to identify the bacterial strains 12RS3 and T5S-T4 as Bacillus circulans and Bacillus altitudinis, respectively. Funding Agency: This Project is financed by INYM with PRASY 0274/17.