INVESTIGADORES
CASTRILLO Maria lorena
congresos y reuniones científicas
Título:
First report of genome sequencing, assembly and gene prediction of Trichoderma koningiopsis.
Autor/es:
CASTRILLO, ML; BICH, GA; FERNANDEZ, T; MEGRIAN, D; AMERIO, NS; SOAREZ, JN; SAPARRAT, MCN; ZAPATA, PD; NAYA, HM; VILLALBA, LL
Lugar:
Posadas
Reunión:
Congreso; 8th Argentinian Congress of Bioinformatics and Computational Biology (CAB2C).; 2017
Resumen:
BACKGROUND:Trichoderma genus comprises a large number of saprotrophic species with a worldwide distribution. They are capable of growing on wood, bark, other fungi and innumerable other substrates demonstrating their high opportunistic potential and their adaptability to different ecological conditions. Members of this genus are important biotechnologically due to their ability to produce a broad spectrum of enzymes and bioactive compounds. The recent advent of genomic and transcriptomic data, combined with insights into the molecular ecology and population genetics of Trichoderma spp., has provided a wealth of information that allows a deeper understanding of this important fungal genus. Currently many species of this genus remain to be characterized physiologically, biochemically, and genetically. In this work, we report the whole-genome shotgun sequencing, assembly and gene prediction of a biotechnological promising Trichoderma koningiopsis POS7 strain.RESULTS:T. koningiopsis POS7 strain was collected from a forest environment of Misiones (Argentina) (27°24?31.8??S, 55°53?48.5??W). Genomic DNA library and draft genome sequencing was performed by Macrogen Co. (Korea) using the Illumina MiSeq platform. Genomic DNA library was prepared using a rapid shotgun library. Quality control procedures removed DNA spike-in, artifacts and ambiguous or low quality reads. Paired ends having at least 90% of bases with quality score greater or equal to Q20 were filtered before assembly.The next-generation sequencing libraries resulted in 7,773,936 paired reads (36,586,254 bp) with an approximate insert size of 100 bp. These sequences were assembled in 248 scaffolds using SPAdes software. The resulting genome sequence of T. koningiopsis POS7 had an estimated size of 36.6 Mb and a GC content of 48.82%. To predict genes in the T. koningiopsis POS7 genome we used ab initio gene predictor, Augustus and FgenesH softwares. These procedures determined that 88 of the 248 scaffolds presented gene prediction and included 11,644 protein-coding genes.CONCLUSION:The Illumina based sequence analysis reveals an approximate genome size of 36.6 Mbp with GC content of 48.82% for T. koningiopsis POS7. Based on ab initio prediction 11,644 coding genes were predicted.