OPTIMIZATION OF A SYNTHETIC MEDIUM FOR CELLULASES SECRETION OF Trichoderma harzianum NATIVE OF MISIONES

CASTRILLO, ML; MARTINEZ, CN; RODRIGUEZ, MD; BICH, GA; ZAPATA, PD; VILLALBA, LL

Mar del Plata

Congreso; - SAIB - 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2015

A reduction in the cost of bioethanol production can be achieved by reducing the cost of raw materials or cellulase enzymes. The aim was to optimize a synthetic culture medium to maximize the cellulases secretion of a Trichoderma harzianum strain native of Misiones. A Box-Behnken response surface design was performed to analyse carboxymethylcellulose (CMC) as sole carbon source; and urea, yeast extract and ammonium sulfate as nitrogen sources. The effects of the factor were tested at three levels and 29 tests were conducted, including a central point quintupled. The synergistic effect of the three cellulases was determined by the filter paper method (FPU). The EGs and CBHs activities were determined by the method of DNS acid, and BGLs activity was determined by the method of p-nitrophenyl-β-glucoside. Taking the days with the highest enzyme activity (without statistically significant differences), a positive influence (statistically significant) of CMC was observed in all the analysed enzymes. Also, it was observed a statistically significant and positive interaction between urea and yeast extract regarding EGs activity; and a statistically significant and positive influence of urea, as well as its interaction with CMC for FPU activity. It was determined which combination of factor?s levels led to the optimization of cellulases?s secretion of a T. harzianum native of Misiones.