IDENTIFICATION OF A FUNGAL STRAIN WITH CELLULOLYTIC CAPACITY, NATIVE OF THE PROVINCE OF MISIONES.

RODRIGUEZ, MD; ALONSO PAIVA, IM; CASTRILLO, ML; ZAPATA, PD; VILLALBA, LL

Rosario

Otro; Reunión SAIB 2014; 2014

Since  several  years,  many  species  of  white  rot  fungi  with  interesting  characteristics  for  use  in  biotechnological applications are being described. Applications of cellulases from new microorganism in industrial and environmental technologies require large amounts of enzymes at low cost. So it is necessary to select and identify new organisms with  elevated  synthesis  of  these  enzymes.  The  aim  of  this  study  was  to  identify  a  fungal  strain  with  cellulolytic capacity   belonging   to  Phylum   Basidiomycota,   with   the   help   of   molecular   and   bioinformatic   tools.   From morphological  identification  in  the  correct  phyllum  of  a  strain  with  cellulolytic  capacity,  DNA  was  extracted  in sufficient  quantity  and  quality  and  ITS1-5,8S-ITS2  regions  were  amplified  and  sequenced using universal  primers ITS 1 and ITS 4. To compare the obtained information with existing databases, the BLASTn (Basic Local Alignment Search Tool) tool of NCBI (National Center for Biotechnology Information) was applied, and contig consensus with the CAP 3 program was performed. 39 sequences were selected to be analyzed phylogenetically with the MEGA 6 program  using  Maximum  Parsimony,  Maximum  Likelihood  and  Neighbor-Joining  methods.  Consequently,  by constructing  monophyletic  clades,  it  was  possible  to  identify  the  fungal  strain  with  cellulolytic  capacity,  native  of Misiones as Pycnoporus coccineus with a very high bootstrap value (>90%).