Bioprospection of white rot fungi isolated in Misiones for lignocellulosic material degradation

CASTRILLO, ML; GONZALEZ, EA; MARTINEZ, CN; FONSECA, MI; ZAPATA, PD; VILLALBA, LL

Iguazu

Simposio; Simposio Internacional de Materiales Lignocelulosicos; 2013

Lignocellulosic material represents the largest source of renewable energy and organic matter of the biosphere. Its structure and composition determines industrial use and its possible application in biotechnology. The white rot fungi (WRF), because of their oxidative and hydrolytic enzymes secretion capacity and distribution, are lignocellulolytic organisms par excellence. The Atlantic Forestor Selva Paranaense, one of the most biodiverse subtropical ecoregions in the planet, is comprised within Atlantic Forest or Atlantic ecoregion complex, so it has great potential for exploration and description of new microorganisms that can reveal innovative capabilitiesfor potential biotechnological applications, area where WRF play an essential role. The aim of our work was to search and isolate WRF, to develop a pool of interest native strains for different industrial applications, such as bioethanol production, pulp and paper industries and bioremediation, among others. Basidiocarps were collected from decaying wood, of different natural ecosystems in Misiones.Samples were stored and transported to the laboratory, where in vitro pure cultures were obtained. It was possible to develop a strain collection of 30 isolated native WRF strains and to keep them under controlled conditions.  This environment will assure stability and genetic and physiological viability, which will allow subsequenttesting for selection in different biotechnological applications.