Utilization of synthetic and semi- synthetic culture media for endo-1,4-β- glucanases secretion by Trichoderma koningiopsis

CASTRILLO, ML; KRAMER, GR; VELAZQUEZ, JE; RODRIGUEZ, MD; BICH, GA; ZAPATA, PD; VILLALBA, LL

Iguazu

Simposio; Simposio Internacional de Materiales Lignocelulosicos; 2013

The ever increasing demand and the environmental concerns together with reducing fossil fuel reserves have played an important role to convert lignocelluloses ethanol production into an attractive research area. The second generation bioethanol emerges as an attractive contribution that efficiently satisfies the latter challenges. To convert lignocellulosic biomass to bioethanol is necessary that the cellulosic components were hydrolyzed to fermentable sugars. Saccharification is a slow and expensive process, and becomes the main economic difficult that hinders the profitable utilization of this abundant energy source. Fungi of Trichoderma genus due to its worldwide distribution and ecological plasticity secrete large amounts of enzymes of industrial interest, such as the cellulases (endo-1.4-β-glucanases, exo-1,4-β-glucanasae y ß-glycosidase), able to degrade and use cellulose and hemicellulose as carbon and energy source. In this work, we compared endo-1.4-β-glucanases enzymatic secretion of Trichodermakoningiopsis native strain of Misiones province, in solid and liquid synthetic medium, with carboxymethylcellulose as carbon source; and semi-synthetic medium, with pine sawdust as carbon source. The results obtained showed that higher values of endo-1.4-β-glucanases were reached when the synthetic medium was used. It could be concluded that pine sawdust seems to be a good candidate for utilization as carbon source in culture media aiming to obtain good enzyme secretion, being an economic and easily available substrate. Increase hydrolytic activity of culture supernatant of these fungi is a way to lower the cost of cellulolytic preparations used in the saccharification stage; and so to develop a viable industrial process, it is essential to establish the parameters and the type of fermentation process to ensure maximum yields.