CASTRILLO Maria lorena
congresos y reuniones científicas
Genes coding xylanase in the Trichoderma koningiopsis POS7 isolate: detection and annotation
CASTRILLO, ML; BICH, GA; VILLALBA, LL; SAPARRAT, MCN; ZAPATA, PD
Congreso; 3rd WOMEN IN BIOINFORMATICS & DATA SCIENCE LA; 2022
Xylan is one of the main components of plant cell mass and is a polysaccharidepolymerized mainly by β-l,4-glycosylic linkage. The sugar unit of xylan is xylose. Theusage of different amounts of xylanases enzymes may enhance the removal of residualxylose of this cell mass. Filamentous fungi are naturally excellent protein secretors andcan produce enzymes in industrially feasible amounts. Our group previously isolatedand selected an isolate of Trichoderma genus coded as T. koningiopsis POS7 by theirpotential biological control and biostimulant mechanisms. Later, was sequenced thegenome of this isolate and assembled it into 248 scaffolds using SPAdes software. Theobjective of the present work was to carry out the detection and the annotation of thegenes coding xylanases in T. koningiopsis POS7. A search for the genes codingxylanases in the T. koningiopsis POS7 genome was performed by bioinformatic tools. Aconsensus sequence obtained by homology from search results applied to variousglobal public access databases was constructed. The short sequences (reads) and theassembled scaffolds that produced gene prediction of the genome of this fungus wereused as raw data. Mapping and assembly was performed using the Geneious 9.1.8bioinformatic program. As a result, four structural coding gene sequences for xylanaseswere detected. Two of these were located in the positive strand composed of 2976 bp(NODE_16) and 5878 bp (NODE_30), and the other two were located in the minusstrand composed of 1093 bp (NODE_3) and 738 bp (NODE_43). The informationobtained let us continue understanding a native biocontrol and biostimulant fungus,and further studies are needed to predict other genes with potential industrialapplications.