In silico prospection of the swollenin gene in the genome of Trichoderma koningiopsis POS7

ROTHÄRMEL FI; BICH, GA; VILLALBA, LL; ZAPATA, PD; CASTRILLO, ML

Modalidad virtual

Congreso; 3rd WOMEN IN BIOINFORMATICS & DATA SCIENCE LA; 2022

The biological control, the use of living organisms (antagonists) to reduce pathogenicactivity, is a promising approach in plant disease management. Also beneficial plant–microbe associations result in more effective nutrient uptake, improved growth anddevelopment, and higher tolerance to biotic and abiotic stresses. Several biocontrol andbiostimulant mechanisms of Trichoderma have been suggested, most of theseprocesses are probably caused by the activation of multiple genes complexes. Throughthe production of Swollenin, Trichoderma may improve the surface area of plant roots,improving their establishment in the rhizosphere. The objective of the present workwas to carry out the prospection and characterization of this gene by bioinformatictools. A search for the swollenin gene in the Trichoderma koningiopsis POS7 genomewas performed by constructing a consensus sequence obtained by homology fromsearch results applied to various global public access databases. The short sequences(reads) and the assembled scaffolds that produced gene prediction of the genome ofthis fungus were used as raw data. Mapping and assembly was performed using theGeneious 9.1.8 bioinformatic program. As a result, a structural coding gene sequencefor the Swollenin protein located in the positive strand composed of 1733 bp, 6 exonsand 5 introns was obtained. This gene had 86.86% identity compared to the same genein Trichoderma asperellum and the information to produce a protein of 483 aminoacids. It is necessary to make a deeper understanding on the genes involved in thebiological control of pathogens and biostimulant mechanisms in order to carry outfurther studies and thus be able to enhance their effects of the antagonistic strains.