Paraguayan Metagenomic analysis of family microflora diversity of native soil

GONZALEZ CORIA, JC; BICH, GA; CASTRILLO, ML

Modalidad virtual

Congreso; 2nd Women in Bioinformatics & Data Science LA Conference; 2021

Microorganisms have been found to play an active role in both animal and plant physiology. Diverse microorganisms live in association with plants, both below and above the ground. The interaction between the microbial populations can have beneficial and detrimental effects on the plant development and growth. The role of Paraguayan microbial communities in native or soils not used in agriculture remains poorly understood and is a field of active concerned. With next-generation sequencing (NGS) it has been possible to explore the microbial diversity by using specific genomic regions. Metagenomics approach can be used to study the microbial diversity in arious contexts in both plants and animals. We used high throughput sequencing to explore the microbial diversity in native un-planted soil samples from the South of Paraguay. NA was extracted from 2.0 g of the soil using the PowerMax Soil DNA Isolation kit (MoBio, Carlsbad, CA, USA) following manufacturer?s instructions. The metagenoma sequencing was performed at Macrogen Korea. Thereafter, the raw sequences of the metagenomas were analyzed using the MG-RAST server. Phylum level distribution across samples were classified in 14 Phyla. The metagenomic analysis of sequences showed Proteobacteria to be the most enriched (46.20%) followed by Actinobacteria (20.77%) and Acidobacteria (8.88) phyla. Forty percent of the total sequences were assigned to the top 11 families of microorganisms. Bradyrhizobiaceae was the most enriched family among the classified families, representing 8.56% of the total assignedsequences, followed by Solibacteraceae (6.99%) and Planctomycetaceae (6.3%). Notably, many members of Bradyrhizobiaceae family contribute to plants in chlorophyll biosynthesis, synthesis of folate and pterines and coenzyme B12 biosynthesis.