INVESTIGADORES
CASTRILLO Maria lorena
congresos y reuniones científicas
Título:
NRPS in the Trichoderma koningiopsis POS7 isolate: detection and annotation
Autor/es:
CASTRILLO, ML; BICH, GA; SOAREZ, JN; VILLALBA, LL; SAPARRAT, MCN; ZAPATA, PD
Lugar:
Modalidad virtual
Reunión:
Congreso; 2nd Women in Bioinformatics & Data Science LA Conference; 2021
Resumen:
One of the mechanisms used by Trichoderma to control invasive pathogens is the production and release of secondary metabolites (SMs) and enzymes. In fungi, the genes responsible for the synthesis of the SMs are often arranged in clusters on the genome, referred to as biosynthetic gene clusters (BGCs). Non-ribosomal peptide synthetases (NRPSs) catalyze the synthesis of a group of SMs, and consist of a series of modules that act as an assembly line. In this work, the presence of BGCs that show gene prediction for the NRPS in T. koningiopsis POS7 was evaluated. Our group previously sequenced the genome of the T. koningiopsis POS7 isolate and assembled it into 248 scaffolds using SPAdes software. From these 248 scaffolds, the BGCs codingfor the NRPSs were searched and identified, using antiSMASH software. In order to corroborate the identity of the BGCs obtained, they were compared against a database for amino acid and nucleotide sequences, using the BLASTp and BLASTn platforms of the NCBI database. Only those BGCs with a complete module necessary to activate and contribute to a single unit to the synthesis of final assembled product were studied. Although in the T. koningiopsis POS7 genome 7 BGCs were identified, only a BGC presented gene prediction for the NRPS. It was located in NODE_62 scaffold between positions 26,611-51,345 bp with a predicted sequence of 7,277 aa. A core peptide biosynthetic gene was detected, which is composed of 5 adenylation domains, 7 peptidyl carrier domains, 5 condensation domains, and 1 reductase terminal domain.Several accessory genes were also detected: 1 additional biosynthetic gene and 4 other genes with incongruous functions for the formation of SMs. These results could facilitate the construction of metabolic engineering strategies to increase the yield of target MSs in Trichoderma species, such as ones catalyzed by NRPS.