A survey on tomato leaf grey spot in the two main production areas of Argentina led to the isolation of Stemphylium lycopersici representatives which were genetically diverse and differed in their virulence

FRANCO, MARIO EMILIO ERNESTO; TRONCOZO, MARIA INÉS; LÓPEZ, SILVINA MARIANELA YANIL; LUCENTINI, GUSTAVO; MEDINA, ROCIO; SAPARRAT, MARIO CARLOS NAZARENO; RONCO, LÍA BLANCA; BALATTI, PEDRO ALBERTO

EUROPEAN JOURNAL OF PLANT PATHOLOGY

SPRINGER

Año: 2017 vol. 149 p. 983 - 1000

0929-1873

Tomato gray leaf spot was first reported inArgentina in 1990. Since then, the disease has not onlyincreased in endemic areas, but also disseminated inother tomato-growing areas. In a survey of plants withtypical symptoms of Tomato grey leaf spot disease weisolated 27 Stemphylium representatives from the twomain tomato-growing areas of Argentina. Cultural featuressuch as sporulation, conidia morphometry amongothers revealed high variability between isolates, whichwas confirmed by Inter Simple Sequence Repeat(ISSR)-PCR technique. A molecular phylogenetic analysiscomprising the Internal Transcribed Spacer (ITS)and the glyceraldehyde-3-phosphate dehydrogenase(gpd) gene partial sequences unambiguously identifiedall isolates as Stemphylium lycopersici. Based on diseaseseverity on detached leaves, isolates were grouped inthree categories (high, medium and low virulent). Nocorrelation was found between phenotypic or genotypiccharacters and the geographical origin of the isolates.