INVESTIGADORES
SAPARRAT Mario Carlos Nazareno
artículos
Título:
Optimizing production of extracellular laccase from Grammothele subargentea CLPS no. 436 strain
Autor/es:
MARIO CARLOS NAZARENO SAPARRAT
Revista:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2004 vol. 20 p. 583 - 586
ISSN:
0959-3993
Resumen:
The production of extracellular laccase by the Grammothele subargentea CLPS no. 436 strain in liquid cultures grown on a carbon-limited basal medium was significantly enhanced when culture conditions, including the addition of CuSO4 5H2O or veratryl alcohol, were consecutively optimized. A laccase activity as high as 1954.5 mU ml-1 of liquid medium was obtained under optimum conditions, which corresponded to non-agitated cultures supplemented with 0.6 mM CuSO4 5H2O. Veratryl alcohol at 1 mM was less effective than CuSO4 5H2O for increasing laccase activity levels; the supplementation of veratryl alcohol resulted only in maximum levels of 44 mU ml-1 in nonagitated cultures.
