Ligninolytic enzyme ability and potential biotechnology applications of the white-rot fungus Grammothele subargentea LPSC no. 436 strain

SAPARRAT, M. C. N.; MOCCHIUTTI, P.; LIGGIERI, C. S.; AULICINO, M.; CAFFINI, N.; BALATTI, P. A.; MARTÍNEZ, M. J.

PROCESS BIOCHEMISTRY

ELSEVIER

Año: 2008 vol. 43 p. 368 - 375

0032-9592

To get a better insight into the ligninolytic system of Grammothele subargentea, extracellular ligninolytic enzyme activities and ability to degrade synthetic dyes as well as Eucalyptus globulus wood were assayed in cultures grown on an agar medium with Cu+2 or dyes and on E. globulus wood chips. Laccase was the only ligninolytic enzyme detected. It was able to decolorize different dyes, being the highest levels of laccase activity in cultures with Brilliant Green. Cultures on wood showed both ligninolytic activity and degradative ability on lipophilic extractives. An extracellular laccase with pI 3.5 and maximal activity at pH 4.0 and 50-55 ºC was detected on liquid cultures containing 0.6 mM Cu+2. The enzyme crude was stable at pH 6.0-7.0 and up to 60 ºC. A laccase-mediator system using G. subargentea laccase crude and 1-hydroxybenzotriazole as mediator improved the tensile strength of a paper from recycled high-kappa-number pulp.