Resveratrol effect on renal osmoprotection: modulation of COX-2 expression

ERJAVEC, LUCIANA CECILIA; PARRA, LEANDRO; FERNÁNDEZ TOME,MARÍA DEL CARMEN; CASALI, CECILIA IRENE

Virtual

Congreso; Reunión Anual de Sociedades de Biociencias; 2020

Soc de Biociencias

Resveratrol (RSV) is a polyphenol present in several plants. Nowadaysit is sold as an over-the-counter dietary supplement mainly dueto its antioxidant, anti-inflammatory and antitumoral effects. Animalrenal injury models describe RSV benefícial effects, while studieswith chronic intake of RSV demonstrate nephrotoxic effects. Hence,RSV effects on renal tissue are still controversial. Due to the urinaryconcentrating mechanism, renal medullary interstitium presents anelevated osmolality that can abruptly vary depending on the hydricstate of the body, reaching values up to 800-1200 mOsm/kg H2O.To survive in this environment, renal cells activate protective pathways.We demonstrated that renal epithelial cells (MDCK) subjectedto high osmolarity undergo an adaptive process during the first 24h,in which the transcription of the osmoprotective gene cyclooxygenase2 (COX-2) is activated, among others. After 48h, these cells arealready adapted and acquire a polarized epithelium morphology. Inthe present work, we evaluate the effect of RSV on adaption and differentiation mechanisms, focusing particularly on COX-2 role. Todo this, MDCK cells were pretreated with different concentrationsof RSV (1, 5, 10, 25μM) and then cultured in hyperosmotic medium(NaCl 125mM, 512 mOsm/kg H2O) for 24 and 48h. Cells werethen harvested to obtain cell number and viability. Immunofluorescence(IF), western blot and RT-PCR analysis were performed. RSVsignificantly decreased cell number in a concentration-dependentmanner at 24 and 48h (p