In vitro and preliminary in vivo assessment of anodized Mg-based alloy as a potential material for biomedical applications.

MERLO, JULIETA; KATUNAR, M.R.; CARRIZO, SABRINA; TANO DE LA HOZ, FLORENCIA

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica.; 2018

Magnesium (Mg) based alloys are promising candidates fordevelopment of new biodegradable materials for fracture repair im-plants. However. the hion dearadation rate of ma in aqueous media releases hydrogen gas, which causes pain and local swelling.Superficial treatments. such as anodization. emerge as a potential solution to this limitation. The aims of this study were to evaluate the1 in vitro citocompatibility o anodized Mo-based material on tibroblasts. and 2) the in vivo osteoconductivity of anodized Mo-basedimplants after 7 days of implantation in a rat model. Anodization ofMg alloy (AZ91) pieces was performed at low voltage for 40 min inpotassium hvdroxide solution. For the obiective 1. bovine fibroblasts adhesion and proliferation were evaluated after 24 h in culture onthe materials (anodized or control -without anodization- AZ91 piec-es. As cellular control. cells crowth was evaluated in culture without material Con a plastic dishl. For objective 2. bone formation stimulation of anodized AZ91 or control -polylactic acid, PLA; a biodegradable material used clinically- was evaluated in adult male rats:two pins of the same material were placed transversely one in eachfemur. At and o days after surgery. different tluorochromes wereadministered intraperitoneally, to be incorporated at mineralizationsites of the new bone. Rats were sacrificed 7 davs after implantationand lemurs were retneved, cleaned, tixed and embedded in poly-methyl methacrylate. Fluorescence and Toluidine blue staining wereobserved on thin sections U um o the implanted areavitro test. an increase in bovine fibroblasts adhesion and prolifera-ton was deleces overine anocizeo sumaces pieces. comozreo itaThe in vivo preliminary results suggest a higher osteoinductivity of the Mg-based pins, compared with PLA pins. In conclu-sion. anodization at low voltage is a promising superticial treatmentfor the develooment of a- based biomedical apolications.