INVESTIGADORES
CARRILLO carolina
artículos
Título:
Phytomonas: A non-pathogenic trypanosomatid model for functional expression of proteins
Autor/es:
MIRANDA MR; SAYÉ M; REIGADA C; CARRILLO C; PEREIRA CA
Revista:
PROTEIN EXPRESSION AND PURIFICATION
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 114 p. 44 - 47
ISSN:
1046-5928
Resumen:
Phytomonas are protozoan parasites from the Trypanosomatidae family which infect a wide variety of plants. Herein, Phytomonas Jma was tested as a model for functional expression of heterologous proteins. Green fluorescent protein expression was evaluated in Phytomonas and compared with Trypanosoma cruzi, the etiological agent of Chagas´ disease. Phytomonas was able to express GFP at levels similar to Trypanosoma cruzi although the transgenic selection time was higher.It was possible to establish an efficient transfection and selection protocol for protein expression.These results demonstrate that Phytomonas can be a good model for functional expression of proteins from other trypanosomatids, presenting the advantage of being completely safe for humans.
