Solid surface fluorescence methodology for fast monitoring of 2,4-dichlorophenoxyacetic acid in seed samples

ALESSO, MAGDALENA; TALIO M. CAROLINA; FERNÁNDEZ L.P.

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017

0026-265X

A new method for pre-concentration/separation and determination of 2,4-dichlorophenoxyacetic acid (2,4-D) by solid-surface fluorescence (SSF) is proposed. Theherbicide was complexed with Rhodamine B at pH 7.0 in the presence of phosphate bufferand anionic surfactant admicelles. A Nylon membrane was selected as a solid support forSSF measurement and the presence of 2,4-D was evident by the RhB quenching effect.Under optimal experimental conditions, the limit of detection and quantification were 6.93and 21 ng L−1, respectively, and the linear range was obtained from 0.021 to 22.11 μg L−12,4-D concentration. The developed methodology showed good sensitivity and adequateselectivity, and it was applied to the 2,4-D determination in seed samples. The SSFrepresents a simple and fast alternative to conventional methods of analysis, employing anexisting instrument in most laboratories.