INVESTIGADORES
ZANETTI Flavia Adriana
artículos
Título:
DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
Autor/es:
CARINA ROMANUTTI; FLAVIA ADRIANA ZANETTI
Revista:
Journal of Veterinary Medicine and Animal Sciences
Editorial:
MedDocs Publishers LLC
Referencias:
Lugar: Reno; Año: 2021 vol. 4 p. 1 - 4
ISSN:
2640-1223
Resumen:
An eukaryotic expression Plasmid (pXL) and a replication-defective human adenovirus serotype 5 (Ad5) carrying the coding sequence of mature VP2 protein from Infectious Bursal Disease Virus (IBDV) were constructed and the evaluation of their immunogenicity was carried out in the murine model. These novel recombinant vectors were able to express VP2 protein in cell cultures and to induce IBDVseroneutralizing antibodies in mice vaccinated with heterologous prime-boost regimen. To our knowledge, this is the first report on the obtaining of a recombinant Ad5 vector expressing VP2 protein of IBDV. Other experiments will be performed to evaluate its usefulness in the chicken, naturalhost of IBDV.
