Microfluidic devices for study the growth of monoclonal antibody producing cells.

MARINA SIERRA RODERO; CRISTIAN PAYÉS; ANA PEÑAHERRERA; CARLOS LASORSA; CLAUDIO L. A. BERLI; BETIANA LERNER; GUSTAVO HELGUERA; MAXIMILIANO PÉREZ

BUENOS AIRES

Congreso; II International Congress in Translational Medicine; 2015

Facultad de Farmacia y Bioquímica y Facultad de Medicina ? UBA

Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlled conditions, as is required for production of therapeutic recombinant proteins. We cultured HEK-293T cells under different experimental conditions. The cells were cultured in polymethyl methacrylate (PMMA) and polydimethylsiloxane (PDMS) microdevices, in the absence or presence of the cell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as the influence of the flow rate have also been tested. Results show that the presence of poly-D-lysine improves the adhesion and viability of the cells in continuous or discontinuous flow. These studies provide insight into the optimal architecture of microchannels for long term culture of adherent cells in order to use microfludics devices as bioreactors for monoclonal antibodies production.